Journal: European Journal of Clinical Microbiology & Infectious Diseases
Article Title: Multiplex PCR to Streptococcus pneumoniae serotype identification directly in cerebrospinal fluid samples
doi: 10.1007/s10096-023-04547-3
Figure Lengend Snippet: Yield of the amplicons using templates with different quality for all master mixes kits tested. In yellow the quantification in nanograms (ng) in the agarose gel made by GelQuant Express—Image Analysis software. Lanes: 1 to 3—Invitrogen™ PCR Supermix Brasil (Thermo Fisher Scientific); 4 to 6—Platinum® Multiplex PCR Master Mix (Thermo Fisher Scientific); 7 to 9—2 × PCR SuperMix (Bio-Helix); 10 to 12—QIAGEN® Multiplex PCR Kit (Qiagen); 13 to 15—GoTaq® Hot Start Green Master Mix (Promega); 16—molecular weight. Templates features: A1—Purified DNA from a serotype 22F strain; A2—DNA extracted from CSF sample with high quality and identified as the presence of serotype 8 strain; A3—DNA extracted from CSF sample with low quality and identified as the presence of serotype 22F strain (same CSF sample from which the strain presented in lanes A1 was isolated). The arrow indicates the 160 bp cpsA gene; The dotted arrow indicates unspecific bands. The table below the figure represents: [ ] ng/µL—concentration in ng of the amplified band correspondent to the genotyping, excluding the band referring to the cpsA gene; total ng—the total amount of produced DNA in ng contained in the total reaction volume correspondent only to the genotyping band
Article Snippet: Then, the amounts of amplicons were quantified after agarose gel by GelQuant Express—Image Analysis software from Thermo Fisher Scientific, to check the molecular weight in nanograms (ng) and bases pair (bp).
Techniques: Agarose Gel Electrophoresis, Software, Multiplex Assay, Molecular Weight, Purification, Isolation, Concentration Assay, Amplification, Produced